P-67: Reactive Oxygen Species, MDA and NO Concentration of Testicular Extract and Their Effect on Sperm Parameters and IVF Outcome in Hyperprolactinemic Adult Male Mice

Background: ROS are free radicals that have a significant role in many of the sperm physiological processes. In the presence of polyunsaturated fatty acids such as sperm plasma membrane,ROS triggers a chain of chemical reactions called lipid peroxidation.The aim of this study was to determine and compare the concentration of MDA and NO in testicular extract of hyperprolactinemic adult male mice to stablish their effect on sperm quality and assess the fertilization potential of spermatozoa and IVF outcome. Materials and Methods: Eighteen mature male mice were divided in 3 groups as control,sham and test. For hyperprolactinemia induction, test group received 40mg/ kg/day sulpiride atypical antipsychotic drug solution , IP for 45 days. Control sham received placebo.45days later spermatozoal suspention was analyzed for sperm parameters and it&#039;s concentration in couda epididymis. For each male mouse, 3 female mice were superovulated, killed and ovums collected. 50000-100000 of taken sperms of each male mouse were injected in macro droplets as each oocyte. After 24 hours the rate of fertilization and 2-cell zygots and after 120 hours rate of necrotic cells, cytoplasmic vesicles in each embryoes and arrested emberyos were investigated and were compered with 2 other group,s data. Additionally, the potential impact of hyperprolactinemia on MDA and NO ratio of testicular tissue were checked and compared with control and sham groups. Results: In hyperprolactinemic group negative correlation was found between testicular extract&#039;s MDA and NO concentration and sperm morphology,vitality and IVF rate.Also,an inverse correlation was found between sprm DNA damage and moyility.Additionaly,2-cell embryos and blastocyst numbers reduced in this group in compare with two other groups.Data showed no significant differences between sham and control&#039;s results (p<0.05). Conclusion: High ROS concentration in testicular extract in hyperprolactinemic mice affects the quality of spermatozoa that consequently dose affect fertilization rate and embryonic quality in IVF cycle.